Plasmid

Part:BBa_K3482025:Design

Designed by: Thierry Marti   Group: iGEM20_UNILausanne   (2020-10-23)


pLPT119 repressilator plasmid(Potvin-Trottier, 2016)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 75
    Illegal EcoRI site found at 831
    Illegal EcoRI site found at 1000
    Illegal EcoRI site found at 1096
    Illegal EcoRI site found at 6309
    Illegal EcoRI site found at 7647
    Illegal EcoRI site found at 8508
    Illegal XbaI site found at 1855
    Illegal XbaI site found at 5546
    Illegal XbaI site found at 6511
    Illegal XbaI site found at 7842
    Illegal SpeI site found at 3870
    Illegal PstI site found at 837
    Illegal PstI site found at 6395
    Illegal PstI site found at 7726
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 75
    Illegal EcoRI site found at 831
    Illegal EcoRI site found at 1000
    Illegal EcoRI site found at 1096
    Illegal EcoRI site found at 6309
    Illegal EcoRI site found at 7647
    Illegal EcoRI site found at 8508
    Illegal SpeI site found at 3870
    Illegal PstI site found at 837
    Illegal PstI site found at 6395
    Illegal PstI site found at 7726
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 75
    Illegal EcoRI site found at 831
    Illegal EcoRI site found at 1000
    Illegal EcoRI site found at 1096
    Illegal EcoRI site found at 6309
    Illegal EcoRI site found at 7647
    Illegal EcoRI site found at 8508
    Illegal BamHI site found at 849
    Illegal BamHI site found at 1117
    Illegal BamHI site found at 5540
    Illegal XhoI site found at 8588
    Illegal XhoI site found at 8669
    Illegal XhoI site found at 8697
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 75
    Illegal EcoRI site found at 831
    Illegal EcoRI site found at 1000
    Illegal EcoRI site found at 1096
    Illegal EcoRI site found at 6309
    Illegal EcoRI site found at 7647
    Illegal EcoRI site found at 8508
    Illegal XbaI site found at 1855
    Illegal XbaI site found at 5546
    Illegal XbaI site found at 6511
    Illegal XbaI site found at 7842
    Illegal SpeI site found at 3870
    Illegal PstI site found at 837
    Illegal PstI site found at 6395
    Illegal PstI site found at 7726
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 75
    Illegal EcoRI site found at 831
    Illegal EcoRI site found at 1000
    Illegal EcoRI site found at 1096
    Illegal EcoRI site found at 6309
    Illegal EcoRI site found at 7647
    Illegal EcoRI site found at 8508
    Illegal XbaI site found at 1855
    Illegal XbaI site found at 5546
    Illegal XbaI site found at 6511
    Illegal XbaI site found at 7842
    Illegal SpeI site found at 3870
    Illegal PstI site found at 837
    Illegal PstI site found at 6395
    Illegal PstI site found at 7726
    Illegal NgoMIV site found at 5322
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Temporary


Source

This plasmid originated from following publication: doi: 10.1038/nature19841.

References